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anti fyn mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti fyn mab
    Anti Fyn Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fyn/Fyn+Antibody/pm41914886-220-6-8
    Average 95 stars, based on 303 article reviews
    anti fyn mab - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Western Blot:

    Article Title: Combinatorial CRISPR screen reveals FYN and KDM4 as targets for synergistic drug combination for treating triple negative breast cancer
    Article Snippet: .. Antibodies used for western blot analysis were: anti-SRC (Santa Cruz biotechnology, mouse sc-5266), anti-FYN (Cell Signaling Technology, rabbit #4023), anti-phospho-FYN Y530 (Invitrogen, PA5-36644), anti-GAPDH (Cell Signaling Technology, rabbit #5174), anti-KDM4A (Bethyl Laboratories, rabbit A300-861A), anti-phospho-ERK T202/Y204 (Cell Signaling Technology, rabbit #9101), anti-ERK (Cell Signaling Technology, rabbit #9102), anti-phospho-STAT3 Y705 (Cell Signaling Technology, rabbit #9145), anti-STAT3 (Cell Signaling Technology, rabbit #4904), anti-phospho-AKT (Cell Signaling Technology, mouse #4051), anti-AKT (Cell Signaling Technology, rabbit #9272), anti-phospho-p38 (Cell Signaling Technology, rabbit #9215), anti-p38 (Cell Signaling Technology, rabbit #8690). .. RNA is extracted from cultured cells with Trizol (Invitrogen) according to the manufacturer’s instructions.

    Article Title: Methods and compositions for the treatment of amyloid-related disorders
    Article Snippet: Immunoblots Proteins were electrophoresed through precast 4-20% tris-glycine gels (Bio-Rad) and transferred with an iBlotTM Gel Transfer Device (Novex-Life Technologies) onto nitrocellulose membranes (Invitrogen). .. Membranes were blocked in blocking buffer for fluorescent western blotting (Rockland MB-070-010) for 1 hour at room temperature and incubated overnight in primary antibodies at 4° C. The following primary antibodies were used: anti-Fyn (Cell Signaling Technology 4023; 1:1,000), anti-phospho-Src (Cell Signaling Technology 2101; 1:1,000). appropriate secondary antibodies were applied for 1 hr at room temperature (Odyssey donkey anti-mouse or donkey anti-rabbit conjugated to IRDye 680 or IRDye 800, LI-COR Biosciences) and proteins were visualized with a LI-COR Odyssey infrared imaging system. ..

    other:

    Article Title: Fyn inhibition by TAE684: A synergistic strategy to suppress melanoma and reverse vemurafenib resistance.
    Article Snippet: Anti-Fyn (4023), anti-pHistone H2A.X (9718), anti-p53(2527), anti-phospho-p53 (Ser33) (2526), anti-p21 (2947), anti-c-Jun (9165), anti-cleaved PARP (9542) and anti-Bax (5023) were from Cell Signaling Technology (CST; MA, USA).

    Article Title: Fyn inhibition by TAE684: A synergistic strategy to suppress melanoma and reverse vemurafenib resistance
    Article Snippet: Anti-Fyn (4023), anti-p-Histone H2A.X (9718), anti-p53(2527), anti-phospho-p53 (Ser33) (2526), anti-p21 (2947), anti-c-Jun (9165), anti-cleaved PARP (9542) and anti-Bax (5023) were from Cell Signaling Technology (CST; MA, USA).

    Incubation:

    Article Title: Triptolide derivative STP1 ameliorates murine systemic lupus erythematosus via targeting Fyn kinase.
    Article Snippet: Triptolide has demonstrated potent immunosuppressive properties in multiple autoimmune disorders, but its severe toxicity has greatly hampered clinical application.. Here, we synthesized a triptolide derivative STP1, which exhibits remarkably reduced toxicity compared with triptolide.. Immune dysfunction plays a critical role in systemic lupus erythematosus (SLE), an archetypical and refractory autoimmune disorder with limited therapeutic options and suboptimal outcomes.

    Article Title: Methods and compositions for the treatment of amyloid-related disorders
    Article Snippet: Immunoblots Proteins were electrophoresed through precast 4-20% tris-glycine gels (Bio-Rad) and transferred with an iBlotTM Gel Transfer Device (Novex-Life Technologies) onto nitrocellulose membranes (Invitrogen). .. Membranes were blocked in blocking buffer for fluorescent western blotting (Rockland MB-070-010) for 1 hour at room temperature and incubated overnight in primary antibodies at 4° C. The following primary antibodies were used: anti-Fyn (Cell Signaling Technology 4023; 1:1,000), anti-phospho-Src (Cell Signaling Technology 2101; 1:1,000). appropriate secondary antibodies were applied for 1 hr at room temperature (Odyssey donkey anti-mouse or donkey anti-rabbit conjugated to IRDye 680 or IRDye 800, LI-COR Biosciences) and proteins were visualized with a LI-COR Odyssey infrared imaging system. ..

    Blocking Assay:

    Article Title: Methods and compositions for the treatment of amyloid-related disorders
    Article Snippet: Immunoblots Proteins were electrophoresed through precast 4-20% tris-glycine gels (Bio-Rad) and transferred with an iBlotTM Gel Transfer Device (Novex-Life Technologies) onto nitrocellulose membranes (Invitrogen). .. Membranes were blocked in blocking buffer for fluorescent western blotting (Rockland MB-070-010) for 1 hour at room temperature and incubated overnight in primary antibodies at 4° C. The following primary antibodies were used: anti-Fyn (Cell Signaling Technology 4023; 1:1,000), anti-phospho-Src (Cell Signaling Technology 2101; 1:1,000). appropriate secondary antibodies were applied for 1 hr at room temperature (Odyssey donkey anti-mouse or donkey anti-rabbit conjugated to IRDye 680 or IRDye 800, LI-COR Biosciences) and proteins were visualized with a LI-COR Odyssey infrared imaging system. ..

    Imaging:

    Article Title: Methods and compositions for the treatment of amyloid-related disorders
    Article Snippet: Immunoblots Proteins were electrophoresed through precast 4-20% tris-glycine gels (Bio-Rad) and transferred with an iBlotTM Gel Transfer Device (Novex-Life Technologies) onto nitrocellulose membranes (Invitrogen). .. Membranes were blocked in blocking buffer for fluorescent western blotting (Rockland MB-070-010) for 1 hour at room temperature and incubated overnight in primary antibodies at 4° C. The following primary antibodies were used: anti-Fyn (Cell Signaling Technology 4023; 1:1,000), anti-phospho-Src (Cell Signaling Technology 2101; 1:1,000). appropriate secondary antibodies were applied for 1 hr at room temperature (Odyssey donkey anti-mouse or donkey anti-rabbit conjugated to IRDye 680 or IRDye 800, LI-COR Biosciences) and proteins were visualized with a LI-COR Odyssey infrared imaging system. ..

    Activation Assay:

    Article Title: Mechanistic insights into PPARγ's role in suppressing collagen-driven platelet activation.
    Article Snippet: The following reagents were used: rosiglitazone and GW9662 (Cat. #71742, 70785; Cayman Chemical, Ann Arbor, MI, USA), 15-deoxyΔ-12,14-prostaglandin J2 (15d-PGJ2) (Cat.#BML-PG050; Biomol, Hamburg, Germany), AEBSF (Cat.#A-540; Gold Biotechnology, St. Louis, MO, USA), aprotinin, glycine, NaCl, NaHCO3, SDS, Tris (Cat. #0332, 0167, 0241, 0335, 0227, 0497) (all from Amresco, Solon, OH, USA), BSA, CaCl2, DMSO (Cat.#A6003-5G, C5670, D8418), EGTA, glucose, (Cat.#E4378, G7021), KH2PO4, Na2HPO4, Na3VO4, Na4P2O7•10H2O, NaF (Cat.#P5655, S9763, S6508, S6422, S7920), prostaglandin E1, sodium citrate, Triton X-100, β-glycerophosphate, β-mercaptoethanol (Cat.#P5515, S4641, T9284, G9422, M3148), von Willebrand factor (vWF) (Cat.#681300) (all from Sigma-Aldrich, St. Louis, MO, USA), citric acid (Cat.#4337; Duksan, Seoul, Korea), EDTA, KCl, leupeptin, MgCl2, Tween-20 (Cat.#75829, 74075, 78436, 75826, 20605) (all from USB, Cleveland, OH, USA), collagen type 1 and ristocetin (Cat.#396) (both from Chrono-Log, Havertown, PA, USA), horseradish peroxidase-conjugated anti-rabbit (Cat.#NA934) or anti-mouse IgG (Cat.#NA931) antibodies (both from Amersham Biosciences, Piscataway, NJ, USA), HEPES (Cat.#11344041; Thermo Fisher Scientific, Waltham, MA, USA), protein A or G sepharose Cl–4B (Cat.#17-096303, 17-0780-01; Cytiva, Marlborough, MA, USA), collagen-related peptide-XL (CRP-XL) (Triple Helical Peptides, Cambridge, United Kingdom), eptifibatide (Cat.# HY-B0686) and H89 (Cat.# HY-15979) (both from MedChem Express, Princeton, NJ, USA). .. The following antibodies were used: anti-PPARγ (E-8, Cat.#sc-7273), anti-phospho-Vav1 (Tyr174, Cat.#sc-16,408-R), anti-Syk (clone LR, Cat. #sc-573), anti-Lyn (clone H6, Cat.#sc-7274), agarose-conjugated antiLyn (44, Cat.#sc-15 AC), anti-linker for the activation of T cells (LAT) (clone 11B.12, Cat.#sc-53550), anti-phospho-PLCγ2 (Tyr753, Cat.#sc101785), anti-PLCγ2 (clone Q20, Cat.#sc-407), agarose-conjugated anti-Fyn (15, Cat.#sc-434 AC), and anti-Btk (clone M138, Cat.#sc-1696) antibodies (all from Santa Cruz Biotechnology, Dallas, TX, USA); antiphospho-Lyn (Tyr397, Cat.#ab226778), anti-phospho-linker for activation of T cells (LAT) (Tyr132, Cat.#ab4476), and anti-Vav1 (Cat. #ab40875) antibodies (all from Abcam, Cambridge, UK); anti-phosphoSyk (Tyr525/526) (clone C87C1, Cat.#2711s), anti-phospho-Syk (Tyr352) (Cat.#2717), anti-Fyn (Cat.#4023), anti-phospho-Src (Tyr416) (Cat.#6943), anti-vasodilator-stimulated phosphoprotein (VASP) (Cat.# sc-46668), anti-phospho-VASP (Ser157) (Cat.# 3111S), and anti-phospho-Lyn (Tyr508, Cat.#2731s) antibodies (both from Cell Signaling Technology, Danvers, MA, USA); anti-phospho-Btk (Tyr551, Cat.#44-1355G, Thermo Fisher Scientific, Waltham, MA, USA); antiprotein tyrosine phosphatase (PTP)RJ (Cat.#MBS649772, MyBioSource, San Diego, CA, USA); anti-phosphotyrosine (clone 4G10, Cat. #05-321), agarose-conjugated rabbit IgG (Cat.#A2909) (both from Sigma-Aldrich, St. Louis, MO, USA); anti-FcRγ (Cat.#06-727, Millipore, Burlington, MA, USA). .. Blood was taken by venipuncture from healthy and drug-free volunteers and collected in tubes containing anticoagulant acid/citrate/ dextrose (22.0 g sodium citrate, 24.5 g dextrose, and 7.3 g citric acid per 1 L) (Cat.#364,816, Becton Dickson, Franklin, NJ, USA).



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